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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 Appu0101 LIBEST_024165 Antheraea pernyi Pupa A full-length cDNA library was conducted by cloning cDNA 다운로드링크 이미지
2 SCRET12B04 LIBEST_024163 Apostichopus japonicus respiratory tree - 다운로드링크 이미지
3 Am0d84SL1 LIBEST_024124 Amoebophrya sp. ex Karlodinium veneficum - cDNA was PCR amplified using a trans-spliced leader primer 다운로드링크 이미지
4 Am1d134SL1 LIBEST_024125 Amoebophrya sp. ex Karlodinium veneficum - cDNA was PCR amplified using a trans-spliced leader primer 다운로드링크 이미지
5 Am2d9SL1 LIBEST_024126 Amoebophrya sp. ex Karlodinium veneficum - cDNA was PCR amplified using a trans-spliced leader primer 다운로드링크 이미지
6 AmGd153SL1 LIBEST_024127 Amoebophrya sp. ex Karlodinium veneficum - After 60 hours of infection host cells were removed by 다운로드링크 이미지
7 FM882258 LIBEST_024150 Amphibalanus amphitrite whole soft tissues - 다운로드링크 이미지
8 AF066866 LIBEST_001323 Arabidopsis thaliana - cell suspension; isolated with polyclonal antibodies against 다운로드링크 이미지
9 1F-40 LIBEST_024067 Agaricus bisporus mycelium The SSH library was constructed with the thermotolerant 다운로드링크 이미지
10 VBL6_10_A01_E001.g1 LIBEST_024435 Arachis hypogaea developing embryo Total RNAs were isolated from developing embryos with Trizol 다운로드링크 이미지
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