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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 SYD1070 LIBEST_011942 Apium graveolens Dulce Group Phloem cDNA library constructed from mRNA purified from isolated 다운로드링크 이미지
2 SYD0676 LIBEST_011944 Apium graveolens Dulce Group Phloem SSH cDNA library constructed from mRNAs purified from 다운로드링크 이미지
3 SYD1065 LIBEST_011943 Apium graveolens Dulce Group Phloem cDNA library constructed from mRNA purified from isolated 다운로드링크 이미지
4 AJ508995 LIBEST_011955 Arabidopsis thaliana - - 다운로드링크 이미지
5 cATI007H03AF LIBEST_011920 Arabidopsis thaliana - Mixed cDNA library of Arabidopsis and E. cichoracearum 다운로드링크 이미지
6 AF325709 LIBEST_011937 Ailuropoda melanoleuca liver - 다운로드링크 이미지
7 As_adfg_01A07_T3 LIBEST_011909 Ascaris suum - Ascaris suum 'large roundworm' is a nematode parasite of 다운로드링크 이미지
8 A2FL820TO LIBEST_018199 Aedes aegypti Pooled eggs, pupae, newly emerged adults, 5-day old adults - 다운로드링크 이미지
9 A1FL001TO LIBEST_018198 Aedes aegypti Pooled eggs, pupae, newly emerged adults, 5-day old adults - 다운로드링크 이미지
10 AcF000183 LIBEST_018137 Asarum caudigerum - - 다운로드링크 이미지
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