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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 Ap-BrVp-I-A10 LIBEST_024240 Acyrthosiphon pisum brain aphids inoculated on one-week old Vicia faba germinations 다운로드링크 이미지
2 sequence LIBEST_001287 Arabidopsis thaliana shoots - 다운로드링크 이미지
3 AF002673 LIBEST_001208 Anopheles gambiae - - 다운로드링크 이미지
4 1A1F LIBEST_024228 Artemia parthenogenetica - Subtractive hybridization was done using total RNA from 다운로드링크 이미지
5 AGESTSEX1 LIBEST_001217 Anopheles gambiae - isolated by differential display of cDNA from male mRNA; 다운로드링크 이미지
6 DK950653 LIBEST_024194 Adiantum capillus-veneris prothallia light-grown 다운로드링크 이미지
7 DK961388 LIBEST_024195 Adiantum capillus-veneris prothallia with plantlets light-grown 다운로드링크 이미지
8 SCINT13E07 LIBEST_024192 Apostichopus japonicus intestine - 다운로드링크 이미지
9 DK944266 LIBEST_024193 Adiantum capillus-veneris young leaves grown in a green house 다운로드링크 이미지
10 SCMSL12H09 LIBEST_024191 Apostichopus japonicus body wall - 다운로드링크 이미지
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