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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 01A01 LIBEST_013845 Arabidopsis thaliana Leaf Senescent rosette leaves #5 and #6 (counted from the bottom) 다운로드링크 이미지
2 AJ802365 LIBEST_013856 Antirrhinum majus whole plant - 다운로드링크 이미지
3 47(2)w-4_04_C02_007 LIBEST_013863 Anopheles funestus - - 다운로드링크 이미지
4 Acmdd19F LIBEST_013889 Ancylostoma caninum - sequences obtained by differential display approach 다운로드링크 이미지
5 Acfdd24F LIBEST_013890 Ancylostoma caninum - sequences obtained by differential display approach 다운로드링크 이미지
6 DD0AB001ZA01FM1 LIBEST_024267 Arabidopsis thaliana cultured cells - 다운로드링크 이미지
7 DD0AA001ZA01RM1 LIBEST_024266 Arabidopsis thaliana cultured cells - 다운로드링크 이미지
8 AangRevC5 LIBEST_024263 Anguilla anguilla Liver Subtracted cDNA library 다운로드링크 이미지
9 AangFor10 LIBEST_024262 Anguilla anguilla Liver Subtracted cDNA library 다운로드링크 이미지
10 Ap-BrOvp-I-A10 LIBEST_024241 Acyrthosiphon pisum brain aphids inoculated on one-week old Vicia faba germinations 다운로드링크 이미지
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