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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 EST001 LIBEST_024453 Ammopiptanthus mongolicus seedling PEG and cold-induced transcript-derived fragment (TDF) 다운로드링크 이미지
2 Aa_aamk2_22C03_T7 LIBEST_024499 Aphelenchus avenae - To obtain mixed-stage nematodes, the nematodes were cultured 다운로드링크 이미지
3 AY401 LIBEST_001014 Antheraea yamamai posterior silkgland Library name stands for ANtheraea Yamamai SilkGland lamda 다운로드링크 이미지
4 EST00001 LIBEST_001033 Arabidopsis thaliana inflorescence apex with young flowers (< ~Stage 9) cDNA fragments from mRNA differential display of the 다운로드링크 이미지
5 subC1 LIBEST_024497 Anopheles gambiae - - 다운로드링크 이미지
6 AS12C LIBEST_024307 Aedes albopictus head, thorax and abdomen DDRT-PCR 다운로드링크 이미지
7 CAFZ1000.rev LIBEST_024311 Alvinella pompejana posterior end The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
8 CAWN385.fwd LIBEST_024315 Alvinella pompejana whole The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
9 CAWI386.rev LIBEST_024314 Alvinella pompejana whole The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
10 CAGB1000.rev LIBEST_024313 Alvinella pompejana posterior end The library was made from oligo(dT)-primed cDNA, cloned into 다운로드링크 이미지
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