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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 CAGA14977.rev LIBEST_024312 Alvinella pompejana posterior end The library was made from oligo(dT)-primed cDNA, cloned into 다운로드링크 이미지
2 CAYO385.rev LIBEST_024319 Alvinella pompejana eggs The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
3 CAYN385.fwd LIBEST_024318 Alvinella pompejana eggs The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
4 CAWP385.fwd LIBEST_024317 Alvinella pompejana whole The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
5 CAWO385.rev LIBEST_024316 Alvinella pompejana whole The library was made from oligo(dT)-primed cDNA and cloned 다운로드링크 이미지
6 pmL3Ac_1 LIBEST_024324 Ancylostoma caninum whole larvae The subtracted library contains ESTs from in vitro 다운로드링크 이미지
7 iL3Ac_1 LIBEST_024323 Ancylostoma caninum whole larvae The subtracted library contains ESTs from infective 다운로드링크 이미지
8 ASH-A002 LIBEST_024326 Anser anser - Reciprocal suppression subtractive hybridization cDNA 다운로드링크 이미지
9 SAH-S004 LIBEST_024327 Anser cygnoides - Reciprocal suppression subtractive hybridization cDNA 다운로드링크 이미지
10 ACGLS14F_C07_.B_050 LIBEST_024339 Amblyomma cajennense - unidirectional library was constructed using long distance 다운로드링크 이미지
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