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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 AJ132743 LIBEST_001633 Arabidopsis thaliana - - 다운로드링크 이미지
2 AF149032 LIBEST_003803 Artemia parthenogenetica - country=China 다운로드링크 이미지
3 AF149034 LIBEST_003804 Artemia parthenogenetica - country=China 다운로드링크 이미지
4 C99851 LIBEST_001741 Arabidopsis thaliana - - 다운로드링크 이미지
5 AB018246 LIBEST_001737 Arabidopsis thaliana - - 다운로드링크 이미지
6 EST01 LIBEST_001725 Arabidopsis thaliana Excised pieces of roots containing nematode feeding sites Templates for the differential-display RT-PCR were 다운로드링크 이미지
7 C99738 LIBEST_001467 Arabidopsis thaliana - - 다운로드링크 이미지
8 C99776 LIBEST_001468 Arabidopsis thaliana - - 다운로드링크 이미지
9 EST143 LIBEST_001492 Arabidopsis thaliana whole plant The plasmid vector, pSMASH, is a yeast expression vector 다운로드링크 이미지
10 AF093608 LIBEST_001517 Acetabularia acetabulum - - 다운로드링크 이미지