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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 AF133720 LIBEST_008863 Amblyomma americanum midgut - 다운로드링크 이미지
2 As_L3_03A03_SKPL LIBEST_008884 Ascaris suum - Ascaris suum is a parasite of pigs. The library was 다운로드링크 이미지
3 IRB1Q3_A05_Q3_034 LIBEST_013922 Anopheles gambiae Abdomens Plasmodium berghei-infected rat blood-fed adult female An. 다운로드링크 이미지
4 RB14_A12_RB14_086 LIBEST_013923 Anopheles gambiae Abdomens Naive Rat Blood-fed adult female An. gambiae mosquitoes were 다운로드링크 이미지
5 S11_H10_S11_089.ab1 LIBEST_013924 Anopheles gambiae Abdomens Sugar-fed adult female An. gambiae mosquitoes were flash 다운로드링크 이미지
6 AEGBR9 LIBEST_008972 Aedes aegypti midgut cDNA fragment was obtained by differential display while 다운로드링크 이미지
7 AEGI27R1 LIBEST_008970 Aedes aegypti midgut cDNA fragment was obtained by differential display while 다운로드링크 이미지
8 AEGI27M2 LIBEST_008971 Aedes aegypti midgut cDNA fragment was obtained by differential display while 다운로드링크 이미지
9 WHE2201_B08_C15ZT LIBEST_013818 Aegilops speltoides Anther Plants were grown in a growth chamber at the University of 다운로드링크 이미지
10 atr01-38ms1-f05 LIBEST_013807 Amborella trichopoda flower buds Amborella trichopoda Baill; This library was made from male 다운로드링크 이미지
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