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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 FE274497_m1166 LIBEST_024358 Ascaris suum - Suppressive-subtractive hybridisation (SSH) library. 다운로드링크 이미지
2 FE274085_f10 LIBEST_024357 Ascaris suum - Suppressive-subtractive hybridisation (SSH) library. 다운로드링크 이미지
3 VBL1_10_A01_E001.g1 LIBEST_024373 Arachis hypogaea - Total RNAs were isolated from leaves and cotyledons with 다운로드링크 이미지
4 hsaa_0001_A01.ab1 LIBEST_024377 Apostichopus japonicus intestine Clontech's SMART cDNA Library Construction Kit (catalog 다운로드링크 이미지
5 c1b10a1.f1 LIBEST_001083 Aspergillus nidulans vegetative mycelia, asexual structures 5' end of cDNA cloned into EcoRI site of pBluescript 3' end 다운로드링크 이미지
6 D001-A1 LIBEST_024296 Acropora millepora - The library was constructed in Lambda Uni-ZAP XR 다운로드링크 이미지
7 33587 LIBEST_001066 Arabidopsis thaliana inflorescence with predominately younger flower buds Flower cDNA library, D. Weigel and associates (reference 다운로드링크 이미지
8 AEMTBA02 LIBEST_001674 Aedes aegypti malpighian tubules and gut The cDNA was cloned into the SalI/NotI sites of pSPORT1. 다운로드링크 이미지
9 AT001240 LIBEST_001684 Acanthamoeba healyi - - 다운로드링크 이미지
10 AJ237012 LIBEST_001614 Aspergillus nidulans - - 다운로드링크 이미지
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