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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 030115KAXA999002HT LIBEST_023198 Actinidia chinensis Fruit tissue including skin Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
2 030115KAZA999002HT LIBEST_023197 Actinidia chinensis Fruit Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
3 AJAD-aaa06e01.g1 LIBEST_021066 Aphanomyces cochlioides mycelium in water on sterile beet slices - 다운로드링크 이미지
4 UMN145 LIBEST_023249 Avena sativa Endosperm - 다운로드링크 이미지
5 A01F-2RAINHA-PL3 LIBEST_026942 Apis mellifera - - 다운로드링크 이미지
6 A01F-2OPERARIA-PL4 LIBEST_026941 Apis mellifera - - 다운로드링크 이미지
7 001107_2736_0895 LIBEST_026940 Amylostereum areolatum Mycelium A vegetatively incompatible interaction (tester) was 다운로드링크 이미지
8 ATCLNL_P1-876845_015_A01 LIBEST_026939 Amblyomma rotundatum - Unidirectional library was constructed using long distance 다운로드링크 이미지
9 AJ01 LIBEST_026935 Apostichopus japonicus Tubefoot Library constrcted with SMART cDNA Library Construction Kit 다운로드링크 이미지
10 CEJ370 LIBEST_026987 Astragalus racemosus whole seedlings - 다운로드링크 이미지