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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 AJAC-aaa13c02.g1 LIBEST_021067 Aphanomyces cochlioides mycelium in potato dextrose broth - 다운로드링크 이미지
2 AJAF-aaa05c12.g1 LIBEST_021068 Aphanomyces cochlioides infected sugarbeet seedlings - 다운로드링크 이미지
3 021203KAWC999002HT LIBEST_023192 Actinidia chinensis Breaking buds Three bud samples were collected 다운로드링크 이미지
4 021127KAWB999004HT LIBEST_023191 Actinidia chinensis Breaking buds Three bud samples were collected from treated and untreated 다운로드링크 이미지
5 021108KAWA999002HT LIBEST_023190 Actinidia chinensis Breaking buds Three bud samples were collected from treated and untreated 다운로드링크 이미지
6 030218KAJB001428HT LIBEST_023196 Actinidia eriantha skin and outer pericarp just below skin Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
7 030115KAYA999002HT LIBEST_023195 Actinidia chinensis Leaf Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
8 030401KAVC007305HT LIBEST_023194 Actinidia chinensis Cell culture Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
9 021210KALB999003HT LIBEST_023193 Actinidia deliciosa Breaking buds Library sequenced by Genesis Research & Development. Library 다운로드링크 이미지
10 030227KAPC999003HT LIBEST_023199 Actinidia eriantha Petals Library sequenced by Genesis Research & Development. 다운로드링크 이미지