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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 FP886016 LIBEST_026425 Acyrthosiphon pisum whole insect Pea aphid whole body full-insert cDNA library constructed by 다운로드링크 이미지
2 ATEST1031 LIBEST_000561 Arabidopsis thaliana - equal quantities of 4 mRNA pools 다운로드링크 이미지
3 A01_AGBL_P1 LIBEST_016975 Anopheles gambiae Salivary gland PCR-based cDNA library made with SMART cDNA library 다운로드링크 이미지
4 AGM_P0_10C LIBEST_016974 Anopheles gambiae Salivary gland PCR-based cDNA library made with SMART cDNA library 다운로드링크 이미지
5 asr11 LIBEST_016962 Aeluropus lagopoides leaf Twenty one-day old seedlings was cultured in 1/2 strength MS 다운로드링크 이미지
6 LIB4-24-B2.bin LIBEST_016942 Amblyomma americanum - Lonestar tick female salivary gland cDNA library 4, 50-200 다운로드링크 이미지
7 a6f09lt.f1 LIBEST_016939 Amblyomma americanum - Lonestar tick female salivary gland cDNA library 1, 50-200 다운로드링크 이미지
8 M4C9.ab1.bin LIBEST_016941 Amblyomma americanum - cDNA library from 3-day feeding ticks constructed by 다운로드링크 이미지
9 TSGL2-10-G1f.bin LIBEST_016940 Amblyomma americanum - Lonestar tick female salivary gland cDNA library 2, unfed 다운로드링크 이미지
10 01B02.abd LIBEST_021074 Ascaris suum - The library was constructed by a suppressive-substractive 다운로드링크 이미지