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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 Dur25.seed.ADAA-aad96g05.b1 LIBEST_026303 Arachis duranensis - Total RNAs were isolated from 6-8 weeks developing seeds of 다운로드링크 이미지
2 Ipa.ADAB-aab69a04.b1 LIBEST_026304 Arachis ipaensis - Total RNAs were isolated from 2 weeks roots of Arachis 다운로드링크 이미지
3 SCII114 LIBEST_000632 Amanita muscaria - - 다운로드링크 이미지
4 OS004 LIBEST_000649 Arabidopsis thaliana - Subtracted cDNA library from salt(NaCl)-treated whole plants 다운로드링크 이미지
5 gooseKBSSH508K01 LIBEST_023869 Anser anser ovary A subtracted cDNA library from the ovaries of laying and 다운로드링크 이미지
6 E05_039, LIBEST_026395 Anguina tritici Whole worm Sequences are obtained from juvenile second stage Anguina 다운로드링크 이미지
7 up8 LIBEST_026386 Apostichopus japonicus body wall - 다운로드링크 이미지
8 ESTA203-1 LIBEST_026389 Angelica sinensis flower buds - 다운로드링크 이미지
9 SSH100 LIBEST_026388 Atriplex centralasiatica seedlings Salt-responsive genes of Atriplex centralasiatica seedlings 다운로드링크 이미지
10 FP901698 LIBEST_026426 Acyrthosiphon pisum whole insect Pea aphid whole body full-insert cDNA library constructed by 다운로드링크 이미지