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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 ASTCTL1 LIBEST_023855 Anopheles stephensi Salivary gland PCR-based cDNA library made with SMART cDNA library 다운로드링크 이미지
2 CDO122.R LIBEST_000620 Avena sativa etiolated leaf A Uni-ZAP XR cDNA library was constructed from etiolated 다운로드링크 이미지
3 clone1 LIBEST_026262 Anopheles sinensis whole body adult females at 24 h post feeding on a Plasmodium yoelii 다운로드링크 이미지
4 Aaitc267 LIBEST_023816 Alternaria alternata Mycelium The mRNA was isolated from Alternaria alternata strains 다운로드링크 이미지
5 AR2 LIBEST_026330 Asparagus racemosus - PCR-based suppression subtractive hybridization was carried 다운로드링크 이미지
6 AC1 LIBEST_026335 Acorus calamus - PCR-based suppression subtractive hybridization was carried 다운로드링크 이미지
7 Dur25.root.ADAA-aad09a08.b1 LIBEST_026302 Arachis duranensis - Total RNAs were isolated from 2 weeks roots of Arachis 다운로드링크 이미지
8 SCCCMX1027E03.b LIBEST_026301 Araucaria angustifolia - - 다운로드링크 이미지
9 Ar1 LIBEST_026309 Ascochyta rabiei - Suppression Subtractive Hybridization 다운로드링크 이미지
10 Ipa.ADAB-aaa01a01.b1 LIBEST_026305 Arachis ipaensis - Total RNAs were isolated from 6-8 weeks developing seeds of 다운로드링크 이미지