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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 ai_SM1000.11 LIBEST_014163 Argopecten irradians adductor muscle - 다운로드링크 이미지
2 M0912797_K090102DIHUA-H12_M13R LIBEST_026672 Aspergillus fumigatus - Suppressive subtractive hybridization (SSH). Aspergillus 다운로드링크 이미지
3 M0912701_NPLAT090902-A12_M13R LIBEST_026671 Aspergillus fumigatus - Suppressive subtractive hybridization (SSH). Aspergillus 다운로드링크 이미지
4 j033 LIBEST_014205 Acetabularia acetabulum whole unicells To reveal differences in gene expression between adult and 다운로드링크 이미지
5 a001-f LIBEST_014204 Acetabularia acetabulum whole unicells To reveal differences in gene expression between adult and 다운로드링크 이미지
6 CR525194 LIBEST_014203 Anopheles gambiae - a directionally cloned and normalized, oligo-T primed cDNA 다운로드링크 이미지
7 Ap138 LIBEST_026690 Agapanthus praecox subsp. orientalis Shoot tips cDNA library was derived from vegetative and reproductive 다운로드링크 이미지
8 BX765120 LIBEST_014202 Anopheles gambiae - a directionally cloned and normalized, oligo-T primed cDNA 다운로드링크 이미지
9 AonE010101P0001-x_A01_phred13_vc LIBEST_026605 Alexandrium ostenfeldii - Including ESTs from different physiological conditions 다운로드링크 이미지
10 ABF10001 LIBEST_023860 Artemisia annua flower - 다운로드링크 이미지