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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 PUC-100 LIBEST_023693 Acyrthosiphon pisum - Suppressive subtractive hybridization (SSH) technology was 다운로드링크 이미지
2 CJ999914 LIBEST_026569 Acropora tenuis full polyps - 다운로드링크 이미지
3 Ac10 LIBEST_014107 Amphidinium carterae - - 다운로드링크 이미지
4 KL3A6 LIBEST_014110 Arabidopsis thaliana - mRNA was prepared from prepared using polyA+ mRNA isolated 다운로드링크 이미지
5 SaltAt29 LIBEST_026544 Aegilops tauschii leaf - 다운로드링크 이미지
6 SaltAt1 LIBEST_026543 Aegilops tauschii leaf - 다운로드링크 이미지
7 AJ568407 LIBEST_014146 Antirrhinum majus petal - 다운로드링크 이미지
8 AJ568031 LIBEST_014145 Antirrhinum majus - - 다운로드링크 이미지
9 PKM015 LIBEST_026590 Antheraea mylitta brain tissues Diapause specific gene of Antheraea mylitta Drury 다운로드링크 이미지
10 PKM018 LIBEST_026591 Antheraea mylitta accessory gland tissue Antheraea mylitta male accessory gland specific gene 다운로드링크 이미지