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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 ATU64965 LIBEST_000859 Arabidopsis thaliana - PRL2 cDNA library, MSU-DOE Plant Research Laboratory, 다운로드링크 이미지
2 AgEST00001 LIBEST_000857 Anopheles gambiae - See 다운로드링크 이미지
3 API65 LIBEST_000878 Allium cepa - Bulb onion library prepared by Native Plants Inc, Salt Lake 다운로드링크 이미지
4 AOB272 LIBEST_000877 Allium cepa Whole leaf For details see Theoretical and Applied Genetics 90 다운로드링크 이미지
5 MF64-5P_G01 LIBEST_023642 Aulacomnium turgidum whole plant Clontech's SMART cDNA Library Construction Kit (catalog 다운로드링크 이미지
6 ADL-P23-D03_1448.E08_08041414O0 LIBEST_023648 Anopheles darlingi - unidirectional library was constructed using long distance 다운로드링크 이미지
7 FS506798 LIBEST_026527 Amynthas koreanus whole body - 다운로드링크 이미지
8 FP527643 LIBEST_026500 Alvinella pompejana ventral tissue This library was constructed by oligo-capping method. Full 다운로드링크 이미지
9 FS996288 LIBEST_026508 Ascidia gemmata intestine - 다운로드링크 이미지
10 1 LIBEST_026507 Azolla caroliniana - - 다운로드링크 이미지