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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 HA-2-1-R1_A01 LIBEST_026496 Arabidopsis thaliana leaf 2 days post inoculation 다운로드링크 이미지
2 RZ_ECtr_5 LIBEST_026470 Actaea racemosa whole rhizome tissues, without root tissues From rhizome of greenhouse-grown black cohosh (Actaea 다운로드링크 이미지
3 BCYL01A01 LIBEST_026471 Actaea racemosa young leaf tissues From young leaf of greenhouse-grown black cohosh (Actaea 다운로드링크 이미지
4 TsiMGovm LIBEST_026475 Anas platyrhynchos magnum - 다운로드링크 이미지
5 F24-2 LIBEST_026478 Adineta ricciae whole body - 다운로드링크 이미지
6 BCRZ201A10 LIBEST_026469 Actaea racemosa whole rhizome tissues, without root tissues From rhizome of greenhouse-grown black cohosh (Actaea 다운로드링크 이미지
7 BCRT101A10 LIBEST_026468 Actaea racemosa whole root tissues, without rhizome tissues From roots of greenhouse-grown black cohosh (Actaea 다운로드링크 이미지
8 A009-A3 LIBEST_026458 Acropora millepora - - 다운로드링크 이미지
9 DD1-5 LIBEST_023610 Arachis hypogaea salt-stressed leaves Partial sequence obtained by DDRT-PCR from salt induced 다운로드링크 이미지
10 DD2-7 LIBEST_023609 Arachis hypogaea drought-stressed leaves Partial sequence obtained by DDRT-PCR from drought induced 다운로드링크 이미지