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The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 AB015096 LIBEST_001509 Arabidopsis thaliana - RIKEN-PMB-FL1 다운로드링크 이미지
2 AT001193 LIBEST_001545 Antheraea yamamai posterior silkgland - 다운로드링크 이미지
3 AB003 LIBEST_003734 Agaricus bisporus Basidiome - 다운로드링크 이미지
4 AB001 LIBEST_003735 Agaricus bisporus Primordium - 다운로드링크 이미지
5 4A3B-AAA-A-01-F LIBEST_003712 Anopheles gambiae - sequenced from the forward priming site that reads from the 다운로드링크 이미지
6 4A3A-AAK-A-02-F LIBEST_003711 Anopheles gambiae - sequenced from forward priming site which reads from the 3' 다운로드링크 이미지
7 AB038710 LIBEST_003792 Arabidopsis thaliana aboveground organ - 다운로드링크 이미지
8 FP561048 LIBEST_026497 Alvinella pompejana pygidum The library consists of purified mRNAs using CloneMiner cDNA 다운로드링크 이미지
9 FP489555 LIBEST_026498 Alvinella pompejana whole body The library consists of purified mRNAs using CloneMiner cDNA 다운로드링크 이미지
10 FP509592 LIBEST_026499 Alvinella pompejana gill This library was constructed by oligo-capping method. Full 다운로드링크 이미지