본문 바로가기 하단 바로가기

The EST division of GenBank, dbEST, is widely used in many applications such as gene discovery and verification of exon–intron structure. However, the use of EST sequences in the dbEST libraries is often hampered by inconsistent terminology used to describe the library sources and by the presence of contaminated sequences. Here, we describe CleanEST, a novel database server that classified dbEST libraries and removes contaminants. We classified all dbEST libraries according to species and sequencing center. In addition, we further classified human EST libraries by anatomical and pathological systems according to eVOC ontologies. For each dbEST library, we provide two different cleansed sequences: ‘pre-cleansed’ and ‘usercleansed’. To generate pre-cleansed sequences, we cleansed sequences in dbEST by alignment of EST sequences against well-known contamination sources: UniVec, Escherichia coli, mitochondria and chloroplast (for plant). To provide user-cleansed sequences, we built an automatic user-cleansing pipeline, in which sequences of a user-selected library are cleansed on-the-fly according to userselected options. The server is available at http://cleanest.kobic.re.kr/ and the database is updated monthly.

Total Count: 1,185

NO. EST Name Library Name Organism Tissue Type Library Info Download Link
1 D5/U10 217 LIBEST_008645 Apis mellifera ovary cDNA fragment cloned from DDRT-PCR screen of ecdysteroid 다운로드링크 이미지
2 QW22 LIBEST_008627 Apis mellifera - Subtractive library 다운로드링크 이미지
3 AB222847 LIBEST_018413 Acropora tenuis whole polyps polyps were inhabited by Symbiodinium spp. strain PL-TS-1 다운로드링크 이미지
4 AF348121 LIBEST_008798 Anopheles gambiae midgut selected by DDRT-PCR from unfed and human blood-fed mosquito 다운로드링크 이미지
5 AF348130 LIBEST_008799 Anopheles gambiae midgut selected by DDRT-PCR on Plasmodium falciparum-infected 다운로드링크 이미지
6 AD01A01T7 LIBEST_006523 Arabidopsis thaliana mixed leaf and root AD Arabidopsis thaliana library enriched for salt-induced 다운로드링크 이미지
7 AD03A02T7 LIBEST_006524 Arabidopsis thaliana mixed leaf and root AD Arabidopsis thaliana subtracted library enriched for 다운로드링크 이미지
8 DQ256368 LIBEST_018549 Arachis hypogaea moisture-stressed leaf - 다운로드링크 이미지
9 DQ256366 LIBEST_018547 Arachis hypogaea leaf - 다운로드링크 이미지
10 NADAQ58TF LIBEST_018204 Aedes aegypti Ibo-11 strain and DS3 strains - 다운로드링크 이미지
1 2 3 4 5 6 7 8 9 10 다음 페이지